Plant Plastid Engineering

Plant Plastid Engineering

Genetic material in plants is distributed into nucleus, plastids and mitochondria. Plastid has a central role of carrying out photosynthesis in plant cells. Plastid transformation is becoming more popular and an alternative to nuclear gene transformation because of various advantages like high protein levels, the feasibility of expressing multiple proteins from polycistronic mRNAs, and gene con...

Plastid genetic engineering via somatic embryogenisis

Engineering the plastid genome of higher plants.

The plastid genome of higher plants is an attractive target for engineering because it provides readily obtainable high protein levels, the feasibility of expressing multiple proteins from polycistronic mRNAs and gene containment through the lack of pollen transmission. A chloroplast-based expression system that is suitable for the commercial production of recombinant proteins in tobacco leaves...

Mediated Plastid RNA Editing in Plant Immunity

Plant regulatory circuits coordinating nuclear and plastid gene expression have evolved in response to external stimuli. RNA editing is one of such control mechanisms. We determined the Arabidopsis nuclear-encoded homeodomain-containing protein OCP3 is incorporated into the chloroplast, and contributes to control over the extent of ndhB transcript editing. ndhB encodes the B subunit of the chlo...

Arabidopsis SAMT1 defines a plastid transporter regulating plastid biogenesis and plant development.

S-Adenosylmethionine (SAM) is formed exclusively in the cytosol but plays a major role in plastids; SAM can either act as a methyl donor for the biogenesis of small molecules such as prenyllipids and macromolecules or as a regulator of the synthesis of aspartate-derived amino acids. Because the biosynthesis of SAM is restricted to the cytosol, plastids require a SAM importer. However, this tran...